FastQCFastQC Report
Thu 30 Jul 2020
HCW3JBGXF_n02_28hr_herm.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCW3JBGXF_n02_28hr_herm.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47480981
Sequences flagged as poor quality0
Sequence length36
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGTTAATGAGGCGAACCGGGGGAACTGAAACATC2674950.5633729429474088No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1859720.39167682740169163No Hit
GGGGGCATTCGTATCATTACGCGAGAGGTGAAATTC1533140.3228956031889906No Hit
TCTCGCGATTCGGAGGGTTTTTGCCCTGCCGAGGAG1437690.30279281719137185No Hit
CTGTTGAGTATGAGATAGTGACTAAAAAAAAAAAAA1322520.27853678928832576No Hit
GGGCGGGACACGTGGTATCCTGTCTGAATATGGGGG1243270.26184589572822853No Hit
GTCGTGATTACCCGCTGAACTTAAGCATATCATTTA1130330.23805952956195237No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGC1027060.21630976832597457No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT1017820.21436372597272157Illumina RNA PCR Primer (100% over 31bp)
GCAGGTTTACCTTGAATAAATCAGAGTGCTCAATAC910200.19169780843407597No Hit
GGGGCATTCGTATCATTACGCGAGAGGTGAAATTCG895660.1886355296660783No Hit
GCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAAT704220.14831622792292348No Hit
GGGGGGACCATCCTCCAAGGCTAAATACTCCTGACT597800.12590304315742762No Hit
TCTGTTACAATGAACGTTCGTTCAAATTTTTGTTTT572600.1205956549212831No Hit
ACCCATATTGATTTTGCAGCTCTGAAAGAAGCGTAT562240.11841372864642372No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGCG387650.027.5892899
GCGAACC388950.027.55185313
TAATGAG390700.027.2763256
GGCGAAC397300.026.97248612
AGGTTAA349200.026.8733272
ATGAGGC402950.026.6388238
AATGAGG405650.026.6125347
GAACCGG405750.026.30019415
CGGGGGA405200.026.16899919
CGAACCG408900.026.1524214
GGGGAAC406500.026.10772521
AGGCGAA410550.026.03248211
GGGAACT411600.025.81325322
AACCGGG426650.025.06778116
GTTAATG425100.024.7438834
AGTGACT249050.024.23068617
TAGGTTA371100.024.0269011
TAGTGAC259000.023.90192216
GTGACTA255200.023.65256118
GGTTAAT445850.023.4567663
Produced by FastQC (version 0.11.5)