FastQCFastQC Report
Thu 30 Jul 2020
HCW3JBGXF_n02_24hr_males.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCW3JBGXF_n02_24hr_males.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70897980
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4112040.5799939575147275No Hit
TCTGTTACAATGAACGTTCGTTCAAATTTTTGTTTT1164630.16426843190736887No Hit
GTCGTGATTACCCGCTGAACTTAAGCATATCATTTA780040.110022880764727No Hit
TCTCGCGATTCGGAGGGTTTTTGCCCTGCCGAGGAG755880.10661516731506314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGGAC96100.022.64522
GGGCCTA38700.022.2452871
ACGTAAG82550.021.78704512
GGCCCGG41000.021.6192531
GACGGCA57900.021.6035211
CGTGGGA90350.021.2314411
GGCCCCG27800.020.7168391
GGTCCCC23550.020.6343691
ACTAAGT134400.020.5361813
GGTTACC99250.020.5230757
GGCCCCA70950.020.3568691
TGCCCGG22850.020.347571
GGCCCCT51650.020.2394891
CACGGCA32650.019.8443871
CGTAAGG92750.019.48831213
GATAGTG109600.019.20209114
GGCCCCC93450.019.0826911
AAAGAGC279400.019.02341330
AACGTAA95700.018.99700211
CTTTGGG108000.018.86155114
Produced by FastQC (version 0.11.5)