FastQCFastQC Report
Thu 30 Jul 2020
HCW3JBGXF_n02_24hr_herm.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCW3JBGXF_n02_24hr_herm.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48735448
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGCGATTCGGAGGGTTTTTGCCCTGCCGAGGAG2890310.5930611328329227No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2338480.4798314360422007No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT2192160.44980811502953655Illumina RNA PCR Primer (100% over 31bp)
CTGTTGAGTATGAGATAGTGACTAAAAAAAAAAAAA1681290.34498297830359537No Hit
GTCGTGATTACCCGCTGAACTTAAGCATATCATTTA1435410.294530995180346No Hit
GGGGGCATTCGTATCATTACGCGAGAGGTGAAATTC902970.18527992191638415No Hit
TCTGTTACAATGAACGTTCGTTCAAATTTTTGTTTT831380.1705904088539414No Hit
GCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT769430.1578789221348699Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGCGGGACACGTGGTATCCTGTCTGAATATGGGGG764510.15686939001771358No Hit
GGGGGTAGTATGGTTGCAAAAAAAAAAAAAAAAAAA726390.14904756800429947No Hit
CGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC719260.14758456719224167Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC674350.13836950878136997Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGGCATTCGTATCATTACGCGAGAGGTGAAATTCG568570.11666456826250986No Hit
CTGTTATTCGTTACCTTTGGAAATAAAATGGTTATT520840.10687087558936567No Hit
GGCGCGAAACTTATCCACTGTTGAGTATGAGATAGT515530.10578131958487384No Hit
GGGATAAAAGCAATATTAATAGAAGTATTTGAATTT510160.1046794522130996No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACC58050.025.03930513
GTGACTA305350.024.68514618
AGTGACT308450.024.40792717
TAGTGAC316900.024.2398116
GCCGAGG464300.024.14135428
GACGGCA42400.024.1247881
CGTTACC128250.023.2159989
CCGAGGA484300.023.21306429
GGCGAAC62500.023.20833812
TGCCGAG487650.023.13296327
ACCTTTG130250.022.7682513
ACGTAAG70900.022.63784812
GGGCCTA32950.022.6227821
TAGATCT320100.022.39693630
TGAGGCG64250.022.2720139
ACTAAGT96700.022.24453713
CGAGGAG517800.022.19238730
CACGGCA26900.022.0794321
GGTATTA66250.022.03096221
AAAGAGC203500.021.96792830
Produced by FastQC (version 0.11.5)