FastQCFastQC Report
Thu 30 Jul 2020
HCW3JBGXF_n02_22hr_males.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCW3JBGXF_n02_22hr_males.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68191232
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4310430.6321091251145016No Hit
TCTCGCGATTCGGAGGGTTTTTGCCCTGCCGAGGAG955150.14006932738801375No Hit
GTCGTGATTACCCGCTGAACTTAAGCATATCATTTA910670.13354649465784693No Hit
TCTGTTACAATGAACGTTCGTTCAAATTTTTGTTTT864530.1267802288716532No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT768980.11276816350817653Illumina RNA PCR Primer (100% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCGG27650.022.2941681
GGCCCGG53850.022.1146141
GGGCCTA43750.021.5633961
ACGTAAG78000.021.38490512
GGCCCCG27900.021.0192451
GACGGCA58200.020.9255871
GGCCCCT55300.020.8024651
GGTCCGG25800.020.695451
GTGACTA148600.020.4813618
GATAGTG150050.020.35344714
CGTAAGG81950.020.29937213
GCACCGG32500.019.9824681
ACTAAGT129050.019.8764213
CACGGCA32250.019.7188111
CGAACCG70600.019.67470424
ATGGGCC259800.019.3533410
GGCCCGC29900.019.2621441
GGTTACC83150.019.1936577
GGTCCCC21700.019.1454071
AAAGAGC265100.019.0816330
Produced by FastQC (version 0.11.5)