FastQCFastQC Report
Wed 29 Jul 2020
HCW3JBGXF_n02_22hr_herm.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCW3JBGXF_n02_22hr_herm.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32274811
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1655360.5128953350028912No Hit
CGTGCAGTGTAAACAACTTTTCAACAAGATCTTTGA1058220.3278779850949398No Hit
TCTCGCGATTCGGAGGGTTTTTGCCCTGCCGAGGAG1031510.31960218140394375No Hit
GTCGTGATTACCCGCTGAACTTAAGCATATCATTTA634310.19653407110579207No Hit
CTGTTGAGTATGAGATAGTGACTAAAAAAAAAAAAA601330.18631557594558804No Hit
TCTGTTACAATGAACGTTCGTTCAAATTTTTGTTTT562420.17425973462710595No Hit
CGCCGGAAGAAAGTAGATGATGAGAATCTATTTATA499640.15480803280304262No Hit
CTGCGCCATCGACGGAGGAGTCTTCTTCGAGGACGG459130.1422564488448902No Hit
GGGGGCATTCGTATCATTACGCGAGAGGTGAAATTC428310.1327072062482411No Hit
GGGCGGGACACGTGGTATCCTGTCTGAATATGGGGG423190.13112082980129613No Hit
ATCGTTCTCAAATTTTGTCACCTGTTCAGCAATTTC413100.12799455277987531No Hit
TGTCATGATTCAGAGCCGACAATCTGTAGAAAAGTT413070.12798525760538149No Hit
TGTGTGTTTTTCGATATTTATCTGTAAATATAAATA386970.11989845579575974No Hit
GCCGGAGAGAAGGGAATCTGTCCAAAGTACTGCGCC367040.11372336154036658No Hit
CCTCTCATCATTCCAATCGTTCTCAAATTTTGTCAC339500.10519039135504155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAGT153400.026.1143912
TGCCCGG17650.025.1524931
CAGTGTA168800.024.9685215
GCGAACC39650.024.8178813
TGCAGTG162050.024.80423
GTAAACA201250.024.543689
AGTGTAA188300.024.2947546
CGTGCAG142100.023.5894321
CGCGGCA16500.023.5423551
TGTAAAC210850.023.4121998
GCCGAGG183000.023.41121528
GTGACTA120300.023.4045718
CACGGCA26650.023.3552721
ACGTAAG49800.023.07275212
GGCGAAC42300.022.97918512
CGTAAGG50200.022.76959613
AGTGACT124100.022.65157917
GGCCCGG20400.022.6440751
CGTGCGG14800.022.5983731
TGAGGCG42500.022.587859
Produced by FastQC (version 0.11.5)