FastQCFastQC Report
Wed 29 Jul 2020
HCW3JBGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCW3JBGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17163037
Sequences flagged as poor quality0
Sequence length15
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAA831690.48458207017790617No Hit
AAAAAAAAAAAACCC427610.24914588251484862No Hit
AAAAAAAAAAAAAAC218110.12708123859431172No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGG551.2217955E-49.0087091
TGGAGCG400.0069956099.0087091
CACGGGG658.332439E-69.0087091
ATACGGC658.332439E-69.0087091
AGAGGGG801.5048863E-79.0087091
GATAGGC801.5048863E-79.0087091
AACCGGG450.00180940929.0087081
ACCGGGG504.6952115E-49.0087081
ATCCGGG603.187234E-59.0087081
CGTAGGC702.1825563E-69.0087081
CTACGGG504.6952115E-49.0087081
ACGTGGG450.00180940929.0087081
GACGGGG853.960122E-89.0087081
TAAGGGC603.187234E-59.0087081
CCGGTAT450.00180940929.0087081
AATAGGC702.1825563E-69.0087081
CTGGGGG504.6952115E-49.0087081
GATCTTT191650.08.905339
CTTCTTT241100.08.899859
ACAGTTT256200.08.8967899
Produced by FastQC (version 0.11.5)