FastQCFastQC Report
Wed 29 Jul 2020
HCW3JBGXF_n01_28hr_males.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCW3JBGXF_n01_28hr_males.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64226769
Sequences flagged as poor quality0
Sequence length15
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTG1106990.17235648269960457Illumina Small RNA Adapter 2 (100% over 15bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG1500.09.001231
CCGTCGC2300.09.001231
ATCCGCG3200.09.001231
TACGCGC5800.09.001231
TTGACCG1600.09.001231
GAACCGG2900.09.001231
CCAACGG3100.09.001231
TAATCCG1450.09.001231
TTAGGCG2700.09.0012291
GTCGCGC5300.09.0012291
TACCCCG1750.09.0012291
TCCGCGG4050.09.0012291
ATTAGCG2250.09.0012291
CTATCGG1650.09.0012291
CGGCATA7150.09.0012291
ATAGCGG3050.09.0012291
GGTCCCG2650.09.0012291
TGAACGG2600.09.0012291
GACCCCG2600.09.0012291
AGCGGGG6600.09.0012291
Produced by FastQC (version 0.11.5)