FastQCFastQC Report
Wed 29 Jul 2020
HCW3JBGXF_n01_28hr_herm.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCW3JBGXF_n01_28hr_herm.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47480981
Sequences flagged as poor quality0
Sequence length15
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTG829020.1746004363304962Illumina Small RNA Adapter 2 (100% over 15bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA2250.09.0010791
TTAGGTA2350.09.0010791
CGTGGGC5750.09.0010791
TTAGGGC3350.09.0010791
AACCGGC3400.09.0010791
GCCTTTA2450.09.0010791
CCTTACG1203.6379788E-129.0010791
AGTCCCG2000.09.0010791
TACCCGG1251.8189894E-129.0010791
TACCCCG1650.09.0010791
CGAACGG2550.09.0010791
TCCGCGG2900.09.0010791
TCCGCGC3100.09.0010791
ATTAGCG1500.09.0010791
GATAACG1300.09.0010791
CTCCGGG2500.09.0010791
CGACGGC4500.09.0010791
ACGTTCG2000.09.0010791
ACTTGGG1700.09.0010791
TTAGCGG2100.09.0010791
Produced by FastQC (version 0.11.5)