FastQCFastQC Report
Wed 29 Jul 2020
HCW3JBGXF_n01_24hr_males.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCW3JBGXF_n01_24hr_males.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70897980
Sequences flagged as poor quality0
Sequence length15
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCGG5050.09.0011421
ATGTGGG5050.09.0011421
GACCGGG4450.09.0011421
GACCGCG2750.09.0011421
TATGCGG4450.09.0011421
CTGCGGG5500.09.0011421
GACGGGC7650.09.0011421
CGAGCGG5500.09.0011421
GACGCCG3150.09.0011421
TGAGCGG3650.09.0011421
GATAGGG3150.09.0011421
AGGTCCG3150.09.0011421
TCCGGCC4550.09.0011411
CGTGGGG7050.09.0011411
AACCGCG3850.09.0011411
GTCGCGG4850.09.0011411
TCTTGCG4300.09.0011411
ACCGGGG4600.09.0011411
CGAACGG3800.09.0011411
TCCGCGG4200.09.0011411
Produced by FastQC (version 0.11.5)