FastQCFastQC Report
Wed 29 Jul 2020
HCW3JBGXF_n01_24hr_herm.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCW3JBGXF_n01_24hr_herm.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48735448
Sequences flagged as poor quality0
Sequence length15
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTG1769680.3631196742051084Illumina Small RNA Adapter 2 (100% over 15bp)
GTGGAATTCTCGGGT621720.12757038778016364No Hit
GCTGGAATTCTCGGG563620.11564888046171239No Hit
CTGGAATTCTCGGGT555580.11399915724587162No Hit
GGACTAGCCTTATTT510260.10469997115857026No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA3200.09.0011391
AACCGGG2650.09.0011391
CACGATA2750.09.0011391
GACCCCG1550.09.0011391
TATTGCG1105.0931703E-119.0011391
TCTACGC2600.09.0011391
GTTCCGG1800.09.0011391
GTATCGG1550.09.0011391
GATTGCG2850.09.0011391
GAACCGG1900.09.0011391
TATACGG901.0588337E-89.0011391
AAAGCGG2650.09.0011391
GGCTCGG3200.09.0011391
CTTACGC2650.09.0011391
ATCTCCG1051.9463187E-109.0011381
ATTAGGG1850.09.0011381
CTCCGGG1350.09.0011381
GGTCGCG2950.09.0011381
GTTTCGG2350.09.0011381
GGGTCGG2450.09.0011381
Produced by FastQC (version 0.11.5)