FastQCFastQC Report
Wed 29 Jul 2020
HCW3JBGXF_n01_22hr_males.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCW3JBGXF_n01_22hr_males.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68191232
Sequences flagged as poor quality0
Sequence length15
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGGG3250.09.0012971
GTCGCGC6500.09.0012971
AGTCCCG1950.09.0012971
TCCGCGG2200.09.0012971
CTCCGCG2050.09.0012971
TGAAGGG3800.09.0012971
CGACGGC5600.09.0012971
CCGGCGG2800.09.0012971
GACCGGG3600.09.0012971
GACCGAA1350.09.0012971
ATAGCGG3100.09.0012971
CCGTGCG2100.09.0012971
TAACGGG2550.09.0012971
TGACGGC5250.09.0012971
CATTCGG1850.09.0012971
TTCCCGC3850.09.0012971
GTAGCGG4200.09.0012971
AAGCCGG1600.09.0012971
AATTGGG3900.09.0012971
CGTAGGG3550.09.0012971
Produced by FastQC (version 0.11.5)