FastQCFastQC Report
Wed 29 Jul 2020
HCW3JBGXF_n01_22hr_herm.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCW3JBGXF_n01_22hr_herm.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32274811
Sequences flagged as poor quality0
Sequence length15
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGC1950.09.00128651
ACAACGC2100.09.00128651
GTTTGGG4700.09.00128651
TTTAGCG1350.09.00128651
CGGGTAA3150.09.00128651
TAGAGGG1800.09.00128651
AGCCGGG2350.09.00128651
CGGCGCG3400.09.00128651
AATCCGG1300.09.00128651
AATCCGC1550.09.00128651
AATCCCG1550.09.00128651
GTACCGC2050.09.00128651
AGTCCCG1850.09.00128651
TACCCGG1600.09.00128651
CAAACCG1550.09.00128651
CGAACGG2500.09.00128651
CGAACGC2950.09.00128651
ACACGCG2450.09.00128651
ATGATTA3900.09.00128651
TCCGCGC3700.09.00128651
Produced by FastQC (version 0.11.5)