FastQCFastQC Report
Mon 22 May 2023
HCV7CBGXT_n01_TF4-MYB33-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV7CBGXT_n01_TF4-MYB33-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935308
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCGGGGTT310133.3158061301731623TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCGGGGGT126891.3566653979223957TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCGGGTTT115811.2382017474457612TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCGGGGAT97461.0420096909253422TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATGGGGGTT92180.9855576986404478TruSeq Adapter, Index 6 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81090.8669871315117588No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATGGGGGGT72580.7760010606131884TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCTCGTAT65880.7043669037365231TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCGGGTAT61180.6541160772708028TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCGCGTAT60350.6452419951502607TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCGCGGTT54630.5840856701749584TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCGCGGAT47240.5050742643065171TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCGCGTTT46450.49662784879419397TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCTGGTTT37360.3994406120764497TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCTGGGTT35540.3799817814024899TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCTGGTAT31560.3374289538847096TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCTCGTTT29270.31294504056417777TruSeq Adapter, Index 6 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCGGGGT26550.2838637112052928TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCGGGTGT26170.2798008784272133TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATGGGGGAT22880.244625299901209TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATGGGGTTT22470.24024171716696532TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCTGGGAT21730.23232988491491574TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATGGGGGGG19860.2123364709806823TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCTCGGAT19000.20314163890397605TruSeq Adapter, Index 6 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18320.19587130656425478No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCGGGGGG17310.18507272470672764TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCTCGGTT14810.15834356169304656TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCGGGGTA14580.1558844786957879TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCGGGGTG13170.1408092307560718TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCGCGGGT11230.12006740025745531TruSeq Adapter, Index 6 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCGGGGG10870.11621840078348522TruSeq Adapter, Index 6 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCGGGTT10850.11600456747937578TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATGGGGGTG9850.10531290227390336TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATGGCGGTT9580.10242615266842581TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATGGGGTAT9360.10007398632322186TruSeq Adapter, Index 6 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)