FastQCFastQC Report
Mon 22 May 2023
HCV7CBGXT_n01_TF4-MYB33-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV7CBGXT_n01_TF4-MYB33-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5677744
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGCCATGGGGGTT235010.41391439980386574TruSeq Adapter, Index 1 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGCCATGGGGGGT126480.22276453464615525TruSeq Adapter, Index 1 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGCCATGGCGGTT91240.16069762919920305TruSeq Adapter, Index 1 (97% over 39bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC82120.14463491133097933No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG79170.1394391857047447No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGCCATGGGGTTT64490.1135838459782618TruSeq Adapter, Index 1 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGCCATGGGGGAT60360.10630983010153329TruSeq Adapter, Index 1 (97% over 39bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA57170.10069140137350328No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGCCATGTCGTAT57170.10069140137350328TruSeq Adapter, Index 1 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGCCATGGCGGAT56810.10005734672081024TruSeq Adapter, Index 1 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)