FastQCFastQC Report
Mon 22 May 2023
HCV7CBGXT_n01_TF2-ABF2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV7CBGXT_n01_TF2-ABF2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5878279
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTATCTCGTAT153510.2611478631755995TruSeq Adapter, Index 6 (97% over 36bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG100850.1715638199547861No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC71510.1216512520076029No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)