FastQCFastQC Report
Mon 22 May 2023
HCV7CBGXT_n01_NLP7-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV7CBGXT_n01_NLP7-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5375744
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCCAATCTCGTAT261510.48646289704271634TruSeq Adapter, Index 23 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCCAATCTCGTTT80740.15019316396018859TruSeq Adapter, Index 23 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCCAATCGCGTAT69310.12893099076146483TruSeq Adapter, Index 23 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)