FastQCFastQC Report
Mon 22 May 2023
HCV7CBGXT_n01_EVRFP-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV7CBGXT_n01_EVRFP-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5623323
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCGAACAATCTCGTAT903561.6068079318936508TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCGAACAATCTCGTTT71380.1269356215177396TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCGAACAATGTCGTAT62530.11119759615444462TruSeq Adapter, Index 12 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCGAACAATCTCGTA59920.1065562124032356TruSeq Adapter, Index 12 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)