FastQCFastQC Report
Mon 22 May 2023
HCV7CBGXT_n01_EV-phd-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV7CBGXT_n01_EV-phd-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3882694
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCACCATCTCGTAT1086562.7984693102263534TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCACCATCTCGTTT263010.6773904922716032TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCACCATGTCGTAT114160.2940226554036965TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCACCATCGCGTAT87640.22571956481762406TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCACCATCTCGTA76350.19664181622347782TruSeq Adapter, Index 7 (97% over 35bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG61090.1573392082919746No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC59850.15414554945612505No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCACCATCTCGGTT55380.14263292445915132TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCACCATCTCGGAT55190.14214357350849693TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCACCATCGCGTTT52600.13547294739168217TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCACCATGTCGTTT51110.13163540572602425TruSeq Adapter, Index 7 (97% over 35bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA38970.10036845551052954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)