FastQCFastQC Report
Mon 22 May 2023
HCV7CBGXT_n01_12G_1-11-NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV7CBGXT_n01_12G_1-11-NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4977887
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATCTCGTAT2859685.744766805674777TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATGTCGTAT181860.3653357338163763TruSeq Adapter, Index 6 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATCTCGTA163050.32754861651138323TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCAGCTCGTAT153470.3083035030726893TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATCTCGTTT147560.2964309957216787TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATCGCGTAT115300.23162438199179694TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATATCGTAT91500.18381293106894553TruSeq Adapter, Index 6 (97% over 37bp)
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT54160.1088011841168753No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT52760.1059887458272958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)