FastQCFastQC Report
Fri 28 Feb 2020
HCV5MBGXF_n02_Salt_NSF_Plate_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV5MBGXF_n02_Salt_NSF_Plate_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371451658
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66638081.7939906462875446No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC5176750.13936537604578414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA868000.019.4913731
GTATCAA2974750.019.078691
GGACCAT1991750.018.4805956
TGGACCA2335350.016.9104295
TTAGTCC851200.016.7535368
ATACCGG1307650.016.5723426
GTTATAC1402000.016.512073
GTCATAT2302150.016.5001451
TAGTCCA897450.016.4983489
GTAGATC1396700.016.01997243
GTACTGG1928150.015.6596671
TCAGGGG1343100.015.33320618
CAACGCA3857500.015.2347664
TACCGGT1449000.015.2109847
CGTTCTA469200.015.13593525
CTGGACC2051500.015.0833774
TACCCAC462000.015.00122638
GTCCACA1026400.014.9874311
GGGGGTA1391750.014.88306121
GTCACGA718450.014.80685133
Produced by FastQC (version 0.11.5)