FastQCFastQC Report
Fri 28 Feb 2020
HCV5MBGXF_n01_Salt_NSF_Plate_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV5MBGXF_n01_Salt_NSF_Plate_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371451658
Sequences flagged as poor quality0
Sequence length20
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG98996442.6651231154283876No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCACC5875550.013.9129962
TACACGT4859550.013.8514122
ACACCTT3769400.013.8436572
CGCATCT4883550.013.8414055
TCAACCG3555300.013.8412441
TCCACCT5926700.013.834473
CTCCTAC3261550.013.8293244
GTCCTCT3786350.013.8288332
CCTCAGT4327950.013.825796
CCACACT3112250.013.8217952
CCTACAA5228900.013.8212171
CCACCTG5933050.013.8208624
CCTCTCT2701700.013.8170614
CCTCTGA3779850.013.8058274
AACCGAT3573950.013.8049453
CGCATCC3045250.013.8040855
TACAAGA5217700.013.8016433
CCTACGT3262350.013.8011366
AACTACC3097250.013.7986126
TCGACCA3000050.013.7973392
Produced by FastQC (version 0.11.5)