FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35670792
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG463660112.998312456869474No Hit
CTCCTCCTCAGCCTTGCGGGTTTCTTCGTCGGCCTTCTCCTTCTTTTCCT987440.2768203184274686No Hit
GCCTCCTCCTCAGCCTTGCGGGTTTCTTCGTCGGCCTTCTCCTTCTTTTC487090.13655149568868558No Hit
GCCCGTTCCCTTGGCTGTGGTTTCGCTAGAAAGTAGTTAGGGACAGTAGG434310.12175507625398393No Hit
GCCTCGGCCTCCTCAACAGAGTCGGTGATGATAATGTTGTCAAAGATCGT428230.12005060050250635No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGG382690.1072838528508142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT951550.066.4583212
GCACACG959150.066.0588611
CACGTCT932950.065.2870714
ACGTCTG944950.064.9507215
ACACGTC947250.064.64712513
CGTCTGA948550.064.5055316
GATCGGA1030400.064.244781
CGGAAGA1050400.064.060314
TCGGAAG1071350.062.5953
CTGAACT976800.062.22831719
ATCGGAA1086000.061.9158942
GAACTCC1021050.059.8187121
AACTCCA1023650.059.4410622
TGAACTC1046500.059.25081320
CAGTCAC792450.058.01186827
AGAGCAC1135400.057.6998448
GTCTGAA1090400.057.10934417
CCAGTCA915450.056.6053426
GAGCACA1156200.056.4349869
TCTGAAC1103100.055.4840718
Produced by FastQC (version 0.11.5)