FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19884013
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT14493607.289071879001487TruSeq Adapter, Index 22 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG417660.21004814269634606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA1671850.067.94144435
CGTACGT1661550.067.9392434
CCGTACG1671300.067.8194933
TACGTAA1661500.067.78750636
TCGTATG1637250.067.6950945
CTCGTAT1624650.067.59753444
CGTATGC1648400.067.5814146
ACGTAAT1657600.067.4212637
GTATGCC1659650.067.1836247
GTAATCT1618250.067.1531639
ACCGTAC1690550.067.1381632
CACCGTA1694200.067.1007731
CGTAATC1641150.067.0798238
TAATCTC1617400.066.8986740
AGTCACC1718250.066.8819328
TATGCCG1653150.066.8465148
TCTCGTA1636450.066.8406343
ACGTCTG1753450.066.746415
CAGTCAC1736150.066.7396827
CGTCTGA1757250.066.6078716
Produced by FastQC (version 0.11.5)