FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15442564
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT187665812.152502654351958TruSeq Adapter, Index 20 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG436490.28265383909045155No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCGCGTAT247660.16037492219556285TruSeq Adapter, Index 20 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTAGCTCGTAT156290.10120728656199839TruSeq Adapter, Index 20 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTTA2124500.068.8290336
GCCTTAT2117650.068.47819537
ACGTCTG2244400.068.30086515
CGTATGC2129600.068.2657346
AGTCACG2217950.068.25539428
GAACTCC2226650.068.25004621
ACTCCAG2223700.068.2369223
CGTCTGA2245400.068.2219116
GTATGCC2133550.068.217247
TATGCCG2118600.068.1881348
ATGCCGT2115150.068.1390649
TCCAGTC2226700.068.0931225
TCGTATG2120500.068.0857745
GTCACGT2215350.068.0646929
CGTGGCC2173250.068.0610733
ACACGTC2255700.068.0600113
CACGTCT2252500.068.042614
GTGGCCT2160150.068.00883534
TGGCCTT2156800.068.0073635
GCACACG2259900.068.00365411
Produced by FastQC (version 0.11.5)