FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16654944
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT11673317.008915791010765TruSeq Adapter, Index 19 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG328100.19699856090780013No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGCTCTCGTAT323260.19409251691269572TruSeq Adapter, Index 19 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAATGATCTCGTAT191240.11482476314540595TruSeq Adapter, Index 19 (97% over 39bp)
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG181740.10912075117154403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1427350.068.1693848
ATGCCGT1429050.068.0174649
CGTATGC1449800.067.36021446
CACGTGA1464400.067.0367831
GTATGCC1458950.066.839247
ACGTGAA1468800.066.7217932
TGCCGTC1455800.066.6378350
GCCGTCT1458600.066.2603651
CTCGTAT1446500.066.203944
CGTGAAA1473950.066.06599433
GTGAAAC1445800.065.9115834
TCGTATG1484000.065.6134745
GTCACGT1505650.065.5703729
CACACGT1535900.065.5001612
TCTCGTA1442750.065.4446543
TGAAACG1457500.065.2768235
CGTCTGA1529150.065.2106216
GCACACG1543650.065.2049311
AAACGAT1397800.065.15337
ACGTCTG1532650.065.1113515
Produced by FastQC (version 0.11.5)