FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18411312
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT3612151.9619188464135529TruSeq Adapter, Index 18 (97% over 40bp)
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG291730.15845149981706896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG454300.067.1938948
ATGCCGT458100.066.5448349
CCGCACA437700.065.71149436
CGTATGC483650.063.1891546
GTATGCC487200.062.79987347
CGCACAT457100.062.72352637
TGCCGTC491150.061.91714550
GCCGTCT496600.060.78690351
CTCGTAT501400.060.1759244
GCACATC478900.060.00714538
TCCGCAC494850.059.62896335
CACATCT483050.059.1943939
CACACGT536300.059.07977312
CGTCTGA539550.058.76362216
GTCACGT532750.058.4620729
TCACGTC528000.058.32499330
GCACACG543100.058.28141811
TCTCGTA514650.058.06220643
TCGTATG527200.057.78203645
GTCTGAA551150.057.60318817
Produced by FastQC (version 0.11.5)