FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20227010
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT11936465.901247885871416TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCTCTCGTAT437580.21633449531097282TruSeq Adapter, Index 16 (97% over 44bp)
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG250550.12386902463587056No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAGCTCGTAT230260.11383788310778509TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1492800.067.9188748
CGTCCCG1453250.067.8994635
CCGTCCC1456050.067.773734
TCACCCG1482600.067.7474130
CCCGTCC1469050.067.7458933
ATGCCGT1495750.067.6772749
CACCCGT1479150.067.6211631
GTCACCC1492450.067.42457629
CCCGATC1369100.067.37446638
GTCCCGA1429100.066.9627836
ACCCGTC1494100.066.7718632
GTATGCC1522850.066.7140747
CGTATGC1527200.066.6849746
AGTCACC1534450.066.51910428
TGCCGTC1523150.066.2804350
CTCGTAT1506400.065.7218444
GCCGTCT1527350.065.6861951
TCGTATG1553800.065.03686545
TCCCGAT1445300.064.9507537
CGTCTGA1617350.064.85699516
Produced by FastQC (version 0.11.5)