FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21116968
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT6921593.277738546556494TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGAT593620.281110432141584TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGTAT499150.2363738961009933TruSeq Adapter, Index 15 (97% over 40bp)
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG447700.21200960289374876No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTTT293870.13916297074466372TruSeq Adapter, Index 15 (97% over 40bp)
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCA218370.10340973192742443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG725400.067.3501948
ATGCCGT724000.066.8519849
GTATGCC779450.065.11809547
GTCACAT1232150.064.4422129
TGTCAGA1205200.064.3587835
ATGTCAG1217200.064.2274834
CGTATGC784400.064.1095746
CACATGT1230900.064.0893731
TGCCGTC744850.064.0267850
ACATGTC1243800.063.2699132
TCACATG1251150.063.15293530
CTCGTAT866650.062.99137544
CATGTCA1253550.062.5716433
GTCAGAA1232900.062.5067136
GCCGTCT709150.062.39864751
TCAGAAT1229400.062.37151737
AGTCACA1293700.061.7879128
CTGAACT1306650.061.7377519
CGTCTGA1311350.061.62085316
TGCGTGA226200.061.49350760
Produced by FastQC (version 0.11.5)