FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20931399
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT7267973.4722810453328994TruSeq Adapter, Index 14 (97% over 44bp)
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG501960.23981196861232257No Hit
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCA245530.11730224052391339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG827950.067.6181948
ATGCCGT831650.067.07326549
TATCTCG844250.066.0757541
GTATGCC863450.065.5351547
TGCCGTC857950.064.6214750
CGTATCT867100.064.54066539
CGTATGC877000.064.2914546
CTCGTAT879750.063.5681144
TCACAGT955200.063.47165330
GCCGTCT854400.063.39905551
GTCACAG961500.063.21957429
GTATCTC883650.063.08600240
TCGTATG901350.061.92050645
TCTCGTA903250.061.58891743
CACAGTT995700.060.6578231
AGTCACA1038050.059.1445828
CCGTATC986100.058.77156438
CCAGTCA1047850.058.7194826
CTGAACT1059750.058.67384319
CTTGAAA885300.058.58637262
Produced by FastQC (version 0.11.5)