FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20904213
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT14269336.82605463310195TruSeq Adapter, Index 13 (97% over 40bp)
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG380110.1818341594586699No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACACTCTCGTAT212190.10150585434620285TruSeq Adapter, Index 13 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTAT209420.10018076260512652TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1633850.067.94189548
ATGCCGT1636950.067.6956349
GTATGCC1667100.066.86796647
TGCCGTC1661300.066.54539550
TCACAGT1707600.066.3941630
GTCACAG1710500.066.3840629
CGTATGC1683900.066.2865246
GCCGTCT1663250.066.04442651
CTCGTAT1666800.065.7429244
CACACGT1770850.065.4065212
TCGTATG1704950.065.2407645
CGTCTGA1771600.065.2060816
ACGTCTG1772700.065.1947615
GCACACG1779100.065.12604511
CTGAACT1775550.065.0215919
CACGTCT1782150.064.91614514
TCTGAAC1782950.064.7911418
GTCTGAA1785950.064.71937617
AGTCACA1764700.064.6988228
TCTCGTA1688050.064.6872543
Produced by FastQC (version 0.11.5)