FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14419269
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC11366137.882597931975608TruSeq Adapter, Index 12 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG244870.1698213688918627No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC215300.14931408797491746TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC213760.14824607266845496TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1323700.068.4335946
ATGCCGT1315300.068.1969447
CACACGT1408000.067.5937412
GTATGCC1346500.067.5931345
GTCACCT1369850.067.530329
GCACACG1411900.067.4338411
TGCCGTC1323000.067.30797648
CGTCTGA1410050.067.3030416
CGTATGC1351300.067.2209444
AGTCACC1383950.067.214528
ACGTCTG1413050.067.19211615
CTCGTAT1298950.067.082142
CACGTCT1418050.066.9354514
GCCGTCT1322900.066.8397249
TGTAATC1299650.066.777636
GTCTGAA1424050.066.74705517
CTGAACT1423300.066.5584619
GTAATCT1294700.066.5355137
AGCACAC1433550.066.4520410
TCGTATG1362600.066.378643
Produced by FastQC (version 0.11.5)