FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12941891
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC5810994.490062541865019TruSeq Adapter, Index 11 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG528840.4086265291525017No Hit
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA132900.10268978466902556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG686650.068.4545146
ATGCCGT691600.067.7363147
GCTACAT667850.067.66556535
GGCTACA682200.067.5453234
ACGGCTA696700.067.52132
CGGCTAC690400.067.4733233
GTATGCC701750.067.109445
CTACATC661650.067.05656436
CACGGCT708350.066.6271431
CGTATGC708350.066.543444
TGCCGTC702850.066.42802448
CACACGT732450.066.1897812
ACGTCTG732650.066.0673915
CGTCTGA734200.065.9133516
GCCGTCT705150.065.8341449
CTCGTAT702500.065.8024742
GCACACG739550.065.5869611
TACATCT679500.065.56827537
TCACGGC726250.065.1972630
TCGTATG725500.065.0767743
Produced by FastQC (version 0.11.5)