FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14690709
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC8579375.839997239071307TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC549630.3741344274125912TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATTC278270.1894190402927456TruSeq Adapter, Index 10 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG236780.1611767001851306No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGGATGC227060.15456027343540737TruSeq Adapter, Index 10 (98% over 50bp)
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA155500.10584921394876178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1010050.068.1279246
TAGCTTA1218850.067.9684734
ATGCCGT962100.067.6304147
ACTAGCT1233250.067.6199432
TATCTCG1042850.067.5179839
GTATGCC1044650.067.1901245
CACTAGC1248150.067.1541231
TCACTAG1254800.067.1387430
GTCACTA1262050.066.8531229
CTAGCTT1240950.066.8285433
AGCTTAT1221650.066.7727235
CTTATCT1079550.066.6333937
TTATCTC1062950.066.5215738
CTCGTAT1007600.066.42020442
CACACGT1290000.066.3438112
CGTATGC1047000.066.31058544
TGCCGTC952350.066.290548
CGTCTGA1289500.066.2403816
ACGTCTG1290750.066.2056215
GCACACG1297850.065.9847911