Basic Statistics
Measure | Value |
---|---|
Filename | HCV3NBGXF_n01_AM75.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 14690709 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC | 857937 | 5.839997239071307 | TruSeq Adapter, Index 10 (100% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC | 54963 | 0.3741344274125912 | TruSeq Adapter, Index 10 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATTC | 27827 | 0.1894190402927456 | TruSeq Adapter, Index 10 (98% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 23678 | 0.1611767001851306 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGGATGC | 22706 | 0.15456027343540737 | TruSeq Adapter, Index 10 (98% over 50bp) |
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA | 15550 | 0.10584921394876178 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 101005 | 0.0 | 68.12792 | 46 |
TAGCTTA | 121885 | 0.0 | 67.96847 | 34 |
ATGCCGT | 96210 | 0.0 | 67.63041 | 47 |
ACTAGCT | 123325 | 0.0 | 67.61994 | 32 |
TATCTCG | 104285 | 0.0 | 67.51798 | 39 |
GTATGCC | 104465 | 0.0 | 67.19012 | 45 |
CACTAGC | 124815 | 0.0 | 67.15412 | 31 |
TCACTAG | 125480 | 0.0 | 67.13874 | 30 |
GTCACTA | 126205 | 0.0 | 66.85312 | 29 |
CTAGCTT | 124095 | 0.0 | 66.82854 | 33 |
AGCTTAT | 122165 | 0.0 | 66.77272 | 35 |
CTTATCT | 107955 | 0.0 | 66.63339 | 37 |
TTATCTC | 106295 | 0.0 | 66.52157 | 38 |
CTCGTAT | 100760 | 0.0 | 66.420204 | 42 |
CACACGT | 129000 | 0.0 | 66.34381 | 12 |
CGTATGC | 104700 | 0.0 | 66.310585 | 44 |
TGCCGTC | 95235 | 0.0 | 66.2905 | 48 |
CGTCTGA | 128950 | 0.0 | 66.24038 | 16 |
ACGTCTG | 129075 | 0.0 | 66.20562 | 15 |
GCACACG | 129785 | 0.0 | 65.98479 | 11 |