FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16010804
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC163361410.203197790691835TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC496850.31032170526851743TruSeq Adapter, Index 9 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG438040.2735902581781652No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGC243560.15212227943081433TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAGCTCGTATGC210460.131448739238829TruSeq Adapter, Index 9 (98% over 50bp)
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA210090.13121764528502128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1896850.068.7047546
ATGCCGT1898700.068.4862347
GTATGCC1911000.068.18439545
TGCCGTC1912400.067.92261548
CGTATGC1925400.067.7562544
GCCGTCT1916200.067.641849
CTCGTAT1864450.067.52717642
CAGATCT1804350.067.2916637
TCGTATG1946200.067.1669843
CGTCTGA1984200.067.063916
ACGTCTG1986600.067.017715
CACACGT1989550.067.0041112
TCTCGTA1853650.066.8764941
GTCACGA1964400.066.7584829
CACGTCT1997450.066.69417614
AGATCTC1822950.066.6914538
TCAGATC1821100.066.6631236
GCACACG2000450.066.6403511
GTCTGAA2001000.066.61317
CTGAACT2000750.066.4885819
Produced by FastQC (version 0.11.5)