FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10826034
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC4334334.003617575928544TruSeq Adapter, Index 8 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG384470.3551346688916735No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC358040.3307212964599963TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC205620.18993104954224233TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC198200.18307720075514267TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGC130780.12080139412087565TruSeq Adapter, Index 8 (98% over 50bp)
TCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAG129680.11978532489367759No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA114820.10605915333352915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG529900.068.2081446
ATGCCGT492200.067.60142547
GTCACAC726850.067.1083329
GTATGCC552950.067.01021645
ACGTCTG753100.066.3319215
AGTCACA741900.066.1957628
CGTCTGA754450.066.1807516
CACACGT755900.066.0673912
CGTATGC556700.065.8861244
GCACACG758300.065.8253711
TGCCGTC488600.065.80015648
ACACTTG737650.065.6326632
CACGTCT761800.065.6022614
CTCGTAT537400.065.4323842
GTCTGAA765500.065.2531717
CAGTCAC760400.065.0280227
ACACGTC770300.064.92319513
CTGAACT766800.064.9146319
GCCGTCT476500.064.7827849
TCTGAAC774150.064.4019918
Produced by FastQC (version 0.11.5)