FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12104353
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC121834010.065304605706723TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC551900.4559516729229559TruSeq Adapter, Index 7 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG264890.2188386277234314No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGGATGC208660.17238426539609347TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAGCTCGTATGC182620.1508713435571484TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATATCGTATGC171830.141957195068584TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATTC168610.13929699505624132TruSeq Adapter, Index 7 (98% over 50bp)
TCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAG151270.12497157014505443No Hit
CCCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAG124700.10302078929786665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1477650.068.3171246
ATGCCGT1428500.068.1165947
GTATGCC1506400.067.9020145
TGCCGTC1412350.067.48299448
CTCGTAT1448900.067.4685842
CACACGT1739400.067.3962612
CAGATCA1653900.067.3757634
ACGTCTG1737350.067.355215
CGTATGC1513450.067.3544544
CGTCTGA1737700.067.3036516
TCACCAG1702300.067.28966530
GCCGTCT1384550.067.217949
GCACACG1743500.067.2033811
CACGTCT1744150.067.1046414
AGTCACC1716900.067.065128
GTCACCA1715550.066.8829929
TCGTATG1471150.066.8526143
GTCTGAA1752250.066.8369417
CTGAACT1746300.066.76977519
ACCAGAT1702250.066.695432
Produced by FastQC (version 0.11.5)