Basic Statistics
Measure | Value |
---|---|
Filename | HCV3NBGXF_n01_AM71.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 13626344 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC | 1070447 | 7.855716837913383 | TruSeq Adapter, Index 6 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 34963 | 0.2565838643145953 | No Hit |
TCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAG | 19599 | 0.14383168368566066 | No Hit |
GCCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTG | 16724 | 0.12273284749012647 | No Hit |
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA | 16614 | 0.12192558767047125 | No Hit |
CCCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAG | 14918 | 0.10947910899651439 | No Hit |
CCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGC | 13876 | 0.10183215688668949 | No Hit |
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA | 13638 | 0.10008554018598093 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 120340 | 0.0 | 68.51783 | 46 |
ATGCCGT | 120095 | 0.0 | 68.27559 | 47 |
TCACGCC | 121365 | 0.0 | 68.01363 | 30 |
CACGCCA | 121495 | 0.0 | 67.84868 | 31 |
GTCACGC | 122855 | 0.0 | 67.79839 | 29 |
GTATGCC | 121710 | 0.0 | 67.77772 | 45 |
TATCTCG | 119960 | 0.0 | 67.76307 | 39 |
TGCCGTC | 121130 | 0.0 | 67.411934 | 48 |
CGCCAAT | 121930 | 0.0 | 67.2593 | 33 |
CTCGTAT | 121590 | 0.0 | 67.19119 | 42 |
CGTATGC | 122945 | 0.0 | 67.125336 | 44 |
GCCAATA | 122010 | 0.0 | 67.097595 | 34 |
GCCGTCT | 121135 | 0.0 | 67.085556 | 49 |
TCGTATG | 124440 | 0.0 | 66.510155 | 43 |
ACGTCTG | 128675 | 0.0 | 66.4056 | 15 |
CGTCTGA | 128595 | 0.0 | 66.37343 | 16 |
ACGCCAA | 124525 | 0.0 | 66.166595 | 32 |
CACACGT | 129290 | 0.0 | 66.05453 | 12 |
ATATCTC | 123505 | 0.0 | 65.869064 | 38 |
TCTCGTA | 123845 | 0.0 | 65.840836 | 41 |