FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13626344
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC10704477.855716837913383TruSeq Adapter, Index 6 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG349630.2565838643145953No Hit
TCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAG195990.14383168368566066No Hit
GCCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTG167240.12273284749012647No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA166140.12192558767047125No Hit
CCCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAG149180.10947910899651439No Hit
CCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGC138760.10183215688668949No Hit
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA136380.10008554018598093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1203400.068.5178346
ATGCCGT1200950.068.2755947
TCACGCC1213650.068.0136330
CACGCCA1214950.067.8486831
GTCACGC1228550.067.7983929
GTATGCC1217100.067.7777245
TATCTCG1199600.067.7630739
TGCCGTC1211300.067.41193448
CGCCAAT1219300.067.259333
CTCGTAT1215900.067.1911942
CGTATGC1229450.067.12533644
GCCAATA1220100.067.09759534
GCCGTCT1211350.067.08555649
TCGTATG1244400.066.51015543
ACGTCTG1286750.066.405615
CGTCTGA1285950.066.3734316
ACGCCAA1245250.066.16659532
CACACGT1292900.066.0545312
ATATCTC1235050.065.86906438
TCTCGTA1238450.065.84083641