FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8413104
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC5748716.8330428341311364TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC716530.8516832788469035TruSeq Adapter, Index 5 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG244430.29053486085516117No Hit
TCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAG138270.16435075567828472No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC133180.1583006700024153TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAGCTCGTATGC115400.13716697190478092TruSeq Adapter, Index 5 (98% over 50bp)
CCCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAG106210.12624353627388893No Hit
CTCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAA97730.116164022220574No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA94480.11230100091476344No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGCTCTCGTATGC91980.10932944606413994TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG778100.068.41830446
ATGCCGT769850.068.1199347
GTATGCC790350.067.8357145
CACACGT864000.067.4378112
ACGTCTG861050.067.38106515
GTCACAC843500.067.334129
CACAGTG838200.067.3089133
CGTCTGA862450.067.2270416
TGCCGTC768850.067.2161848
CACACAG842450.067.1887331
GCACACG867600.067.1821911
CGTATGC796650.067.07120544
CACGTCT867350.066.955814
AGTCACA856550.066.9137628
CTCGTAT692450.066.9046542
ACACAGT845800.066.8812332
GCCGTCT762250.066.8155949
GTCTGAA870450.066.6373417
TCACACA852000.066.58830
ACACGTC874450.066.5558613
Produced by FastQC (version 0.11.5)