FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11391648
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC7007796.151691133714806TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC329750.28946645823326TruSeq Adapter, Index 4 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG179050.1571765560171803No Hit
TCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAG134630.11818307588155813No Hit
CCCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAG122670.10768415597111147No Hit
CAGCTTTGCAACCATACTACCCCCGGAACCGAAAGACTTTCGTTTCCGGA115080.10102137987409723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG880050.067.1980446
ATGCCGT882000.066.9026547
GTATGCC890050.066.53989445
ACTGACC874350.066.1624932
TGCCGTC891650.065.9430848
CTGACCA874850.065.8526333
CGTATGC903650.065.6740244
AGTCACT917950.065.6515828
CTCGTAT855300.065.61381542
GCCGTCT893700.065.56467449
CGTCTGA930000.065.5431716
ACGTCTG930650.065.5234315
CACACGT931200.065.480412
ACCAATC813700.065.3344236
GCACACG933400.065.3061611
CACGTCT934950.065.1886714
TCACTGA903450.065.1595630
GACCAAT861000.065.15562435
GTCACTG922350.064.985529
TCGTATG914600.064.91070643
Produced by FastQC (version 0.11.5)