FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12262155
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC166646713.59032731196107TruSeq Adapter, Index 3 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG635100.5179350611699167No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAGCTCGTATGC394150.3214361586523739TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC337940.2755959291005537TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATATCGTATGC267680.21829768095412266TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATTC171590.13993461997503703TruSeq Adapter, Index 3 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1941450.068.43516546
ATGCCGT1914100.068.2318147
ACTTAGG2081600.068.1578932
GTATGCC1964900.068.1303145
CACTTAG2089050.068.1242531
TCACTTA2097200.068.0870230
CTTAGGC2070650.068.06196633
CTCGTAT1906650.067.8700842
TGCCGTC1896900.067.839448
CGTATGC1968550.067.7888744
GCCGTCT1878350.067.6205849
TTAGGCA2063900.067.6076934
AGGCATC1950600.067.5235636
TCGTATG1946800.067.4316143
GTCACTT2119650.067.41872429
AGTCACT2121550.067.4064628
TCTCGTA1878950.067.3528441
CGTCTGA2148400.067.2697116
ACGTCTG2150550.067.2388115
CACACGT2154400.067.1809112
Produced by FastQC (version 0.11.5)