Basic Statistics
Measure | Value |
---|---|
Filename | HCV3NBGXF_n01_AM67.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 14847770 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC | 1781218 | 11.99653550667878 | TruSeq Adapter, Index 2 (100% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC | 52655 | 0.35463237913841605 | TruSeq Adapter, Index 2 (98% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 29819 | 0.2008315053371651 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATATCGTATGC | 26979 | 0.18170405387475697 | TruSeq Adapter, Index 2 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATTC | 26571 | 0.17895616648156593 | TruSeq Adapter, Index 2 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAGCTCGTATGC | 22727 | 0.15306675682610923 | TruSeq Adapter, Index 2 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATGC | 20953 | 0.1411188346802247 | TruSeq Adapter, Index 2 (98% over 50bp) |
GCCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTG | 15326 | 0.1032208877157984 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 205295 | 0.0 | 68.63819 | 46 |
ATGCCGT | 199775 | 0.0 | 68.44095 | 47 |
TATCTCG | 204415 | 0.0 | 68.325165 | 39 |
GTATGCC | 208880 | 0.0 | 68.231674 | 45 |
CTCGTAT | 204040 | 0.0 | 67.845 | 42 |
TGCCGTC | 197940 | 0.0 | 67.80764 | 48 |
GTATCTC | 206975 | 0.0 | 67.75401 | 38 |
GTCACCG | 227430 | 0.0 | 67.72062 | 29 |
CGTATGC | 209550 | 0.0 | 67.714554 | 44 |
TGTATCT | 208580 | 0.0 | 67.71088 | 37 |
ATGTATC | 217040 | 0.0 | 67.65812 | 36 |
GCCGTCT | 194905 | 0.0 | 67.620895 | 49 |
AGTCACC | 229175 | 0.0 | 67.54095 | 28 |
CACCGAT | 227050 | 0.0 | 67.51155 | 31 |
TCACCGA | 227670 | 0.0 | 67.50933 | 30 |
ACCGATG | 226875 | 0.0 | 67.42787 | 32 |
CGTCTGA | 233155 | 0.0 | 67.353455 | 16 |
TCGTATG | 206085 | 0.0 | 67.341606 | 43 |
ACGTCTG | 233440 | 0.0 | 67.300156 | 15 |
CCGATGT | 226180 | 0.0 | 67.285355 | 33 |