FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14847770
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC178121811.99653550667878TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC526550.35463237913841605TruSeq Adapter, Index 2 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG298190.2008315053371651No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATATCGTATGC269790.18170405387475697TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATTC265710.17895616648156593TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAGCTCGTATGC227270.15306675682610923TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATGC209530.1411188346802247TruSeq Adapter, Index 2 (98% over 50bp)
GCCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTG153260.1032208877157984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2052950.068.6381946
ATGCCGT1997750.068.4409547
TATCTCG2044150.068.32516539
GTATGCC2088800.068.23167445
CTCGTAT2040400.067.84542
TGCCGTC1979400.067.8076448
GTATCTC2069750.067.7540138
GTCACCG2274300.067.7206229
CGTATGC2095500.067.71455444
TGTATCT2085800.067.7108837
ATGTATC2170400.067.6581236
GCCGTCT1949050.067.62089549
AGTCACC2291750.067.5409528
CACCGAT2270500.067.5115531
TCACCGA2276700.067.5093330
ACCGATG2268750.067.4278732
CGTCTGA2331550.067.35345516
TCGTATG2060850.067.34160643
ACGTCTG2334400.067.30015615
CCGATGT2261800.067.28535533