FastQCFastQC Report
Tue 3 Mar 2020
HCV3NBGXF_n01_AM66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCV3NBGXF_n01_AM66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13440813
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC9251946.883467540244776TruSeq Adapter, Index 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG308760.22971824695425788No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC275050.20463791885208135TruSeq Adapter, Index 1 (98% over 50bp)
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA195690.1455938714421516No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATGC187640.13960465040321593TruSeq Adapter, Index 1 (98% over 50bp)
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA167480.12460555771440314No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGCTCTCGTATGC165640.12323659290550357TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1098050.068.1495746
ATGCCGT1098900.067.8832147
GTATGCC1110900.067.3605145
TGCCGTC1110850.066.9985748
CACACGT1153000.066.9288812
GCACACG1155250.066.82857511
ACGTCTG1152300.066.8123415
CGTCTGA1153950.066.71377616
CGTATGC1124150.066.59794644
AGTCACA1140450.066.5178828
GTCACAT1138900.066.5100829
CTCGTAT1083550.066.4387442
CACGTCT1160800.066.4158414
GCCGTCT1118600.066.36230549
ACATCAC1119450.066.29265632
GTCTGAA1163550.066.2174917
CTGAACT1167850.065.7998619
CATCACG1125900.065.74551433
TCGTATG1139900.065.7148443
ACACGTC1178450.065.5220813
Produced by FastQC (version 0.11.5)