FastQCFastQC Report
Fri 14 Feb 2020
HCTNJBGXF_n02_Salt_NSF_Plate_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTNJBGXF_n02_Salt_NSF_Plate_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190251718
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG151091207.941647076217204No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT2471880.12992681621934157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1207900.020.6913641
GGACCAT973300.019.305956
GGTATCA432200.018.1901021
TGGACCA1122250.017.8567735
GTTATAC865200.017.7841193
GTAGATC697900.017.63349243
ATACCGG845050.017.5971626
GTACTGG883250.017.5727711
GGGGGTA823250.017.18218221
TTAGTCC249500.017.1401548
TCAGGGG834300.016.9759618
TAGTCCA265150.016.949929
GTCATAT912250.016.9418031
GGGGTAT814750.016.81282822
TACCGGT906500.016.77817
TAGATCT731000.016.71457344
GGTATTC827450.016.52503424
TACTGGA931100.016.5093212
GGGTATT835100.016.43442323
CATGTGG1174300.016.3771210
Produced by FastQC (version 0.11.5)