FastQCFastQC Report
Fri 14 Feb 2020
HCTNJBGXF_n01_Salt_NSF_Plate_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTNJBGXF_n01_Salt_NSF_Plate_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190251718
Sequences flagged as poor quality0
Sequence length20
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG176780369.29191924563856No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCTT2325050.013.8866772
TCCTTAC2324900.013.8861594
CCTTACG2325550.013.8741455
TTCCACC1154750.013.8610452
AACCGAT1618600.013.8601263
TCAACCG1617900.013.8538871
CCACTCT1500350.013.8411685
CCTCAGT1659200.013.8395366
CCTCTCT886500.013.8352394
CTCCTAC1208250.013.8339984
GAATCCT2337600.013.8218031
TCTCGAT1989400.013.812736
CCACACT1136800.013.8126362
CTTACGC2334350.013.8074586
TCGACTT1425200.013.8072496
AACTGCA2127050.013.8067383
TCGACCA1216300.013.8062
TACACCT990400.013.8010721
AGACATC1592150.013.7928362
CCTACGT1211950.013.7888796
Produced by FastQC (version 0.11.5)