FastQCFastQC Report
Wed 26 Feb 2020
HCTL5BGXF_n02_Salt_NSF_Plate_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTL5BGXF_n02_Salt_NSF_Plate_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352375972
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67605641.9185655485045388No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC4050590.1149508003343656No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT3710580.10530173152668877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2796250.018.9086151
GGACCAT2109650.018.7622576
GGTATCA847100.018.7413651
GTAGATC1731100.018.35799443
TAGATCT1745200.017.61139744
TTAGTCC771550.017.2502128
TGGACCA2479350.017.1252675
TAGTCCA809000.017.1108579
GTTATAC1505350.016.7848033
ATACCGG1426950.016.6780646
GTACTGG1925450.016.2827641
GTCATAT2020150.015.5695091
ACTGGAC1933950.015.5629563
TACCGGT1580650.015.4978857
TCAGGGG1469300.015.44436518
GGGGGTA1489100.015.36271421
CTGGACC2143000.015.3359254
CATGTGG2636750.015.29770610
GTCCACA942050.015.29526311
ATTAGTC896000.015.250297
Produced by FastQC (version 0.11.5)