FastQCFastQC Report
Wed 26 Feb 2020
HCTL5BGXF_n01_Salt_NSF_Plate_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTL5BGXF_n01_Salt_NSF_Plate_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352375972
Sequences flagged as poor quality0
Sequence length20
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG101699482.8861071151582376No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTAC3676300.013.8590034
CGCATCC4434900.013.8492615
GGTCTTG11448500.013.8486961
AATCCTT3970650.013.8393852
TCAACCG3514650.013.8273851
TCGACCA3486800.013.8226962
CCTACGT3674500.013.8212166
TCCTTAC3984400.013.8155834
TCTTGTG11446450.013.8060073
GTCTTGT11466800.013.8029262
TCGCATC4448150.013.8023444
CCTATAG4368200.013.8019372
TGTGTTC11619150.013.8019226
TATAGCA4345150.013.8018574
GCCTATA4368450.013.7975391
TCCTACG3690300.013.7929585
CCTTACG3986850.013.791825
AACTACC2678900.013.7891796
TCTCCTA3699600.013.7881473
CCACACT2940300.013.7878062
Produced by FastQC (version 0.11.5)