FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25733935
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13430665.219046368151625No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA278380.10817622722681161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG44800.043.27241511
ACACGTC48900.040.57414213
ACGTCTG48700.040.23787715
CACACGT48650.040.20730612
CGTCTGA53700.036.62166216
CACGTCT54200.036.2191914
AGCACAC57400.034.87106710
AGAGCAC65500.030.8260088
CAGTCAC55000.027.16733727
TCGGAAG76000.026.6651953
CTCCAGT68350.026.11060524
CTGAACT76250.026.06665819
CCAGTCA60750.025.7480326
CGGAAGA93000.022.5432724
ACTCTAG64650.022.3051935
GATCGGA93950.021.50381
ATCGGAA96350.021.2831882
GCTGACT39800.019.9322721
CTAGCAG45400.018.5756478
GAACTCC104550.018.27474821
Produced by FastQC (version 0.11.5)