FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE9-bZIP63-RFP-Root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE9-bZIP63-RFP-Root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15001687
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT304750.2031438197584045No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC265210.17678678404635426No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC200480.13363830347880207No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG196140.13074529551243136No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA196130.13073862959545815No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG185930.12393939428278966No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC163630.10907439943254381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG136900.029.3543575
GCTGACT79150.028.3944021
TACTCTA66500.027.0040174
GTACTCT71300.024.4494783
CTAGTTG73350.024.0963658
CTAGCAG95250.023.9208348
CTGACTC95000.023.3982982
TCTAGTT78650.022.9623897
TGTACTC75500.022.9505122
CTAATGC75050.022.75767921
GACTCTA104200.022.7433094
GGACACC76150.021.92322529
AGGACTG88150.021.678562
ACCTCTA79600.021.6324917
GACACCT78700.021.6130530
TTGTACT79950.021.5862451
CTCTAGT86650.020.9669086
GTTACCT81150.020.9604614
GGACTGT93700.020.5066463
ATGCTGG83500.020.45472724
Produced by FastQC (version 0.11.5)