FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE8-XERIR0-GFP-Root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE8-XERIR0-GFP-Root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12881925
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT259500.20144504800330693No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT155870.12099899665616745No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC150880.11712535199514047No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA132040.10250020862565183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG121650.032.3430525
TACTCTA68800.029.9164874
GCTGACT68450.028.3830971
GTACTCT76450.026.5563773
CTAGTTG75550.026.4988888
TCTAGTT82900.025.3743237
CTAGCAG76650.025.1140428
CTCTAGT84100.024.6407626
GACTCTA80100.024.6035394
CTAATGC80750.024.09877421
GGACACC79200.023.90725529
ACCTCTA82800.023.84010117
CTGACTC84250.023.4334352
GACACCT84400.022.51723530
GTTACCT91200.021.87464714
ATGCTGG90950.021.70388224
TACCTCT93800.021.38033316
AGGACTG101600.020.84103662
TGGACAC92650.020.73884428
CCTCTAA96150.020.67561118
Produced by FastQC (version 0.11.5)