FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE7-AL7-RFP-Root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE7-AL7-RFP-Root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10397421
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA228390.2196602407462389No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA209680.20166539375485515No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT186220.17910210618575512No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT142600.13714939502786316No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC127970.12307859804849683No Hit
GTCTTCTTCTGCATTACGGGGCCGTCGGAGGGGAAGTTGGTGCCGCGCAG121750.11709634533409775No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT117300.11281643784550033No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT112790.10847882373907915No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA109790.10559349284788987No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG106120.10206377139100166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA85550.037.809594
TTGTACT87950.036.6210861
ACTCTAG118850.036.258425
TGTACTC89650.036.198442
GTACTCT90700.035.7013323
CTCTAGT98550.033.5327766
TCTAGTT98600.033.048347
CTAGTTG97800.032.887958
CGTATGC21450.032.47011646
ACCTCTA103700.030.88149617
GTATGCC23500.030.23320447
GGACACC102400.030.21386129
CTAATGC104950.030.2138421
GTTACCT108200.029.59714714
GCACACG24800.029.49529311
TATGCCG23650.029.44935848
GACACCT105200.029.3764230
TAGTTGT111250.029.0689969
TACCTCT110850.028.88959116
TTACCTC111750.028.62560315
Produced by FastQC (version 0.11.5)